GC-MS Analysis of Bioactive Components from Syzygium Aromaticum and its Inhibition to Pathogenic Staphylococcus Sciuri Obtained from Aquatic Sources

Authors

• Seham Abdel-Shafi, Department of Botany and Microbiology, Faculty of Science, Zagazig University, Zagazig, Egypt AND Al- Farahidi University, Baghdad, IraqFollow
• Mostafa I. Abdelglil, Al- Farahidi University, Baghdad, Iraq
• Mohamed F Ghaly, Department of Botany and Microbiology, Faculty of Science, Zagazig University, Zagazig, Egypt
• Saida Amer, Department of Botany and Microbiology, Faculty of Science, Tanta University,Tanta, Egypt
• Sobhy El Sawy Elsilk, Department of Botany and Microbiology, Faculty of Science, Tanta University,Tanta, Egypt
• Maysa M. Mohamed, Al- Farahidi University, Baghdad, Iraq AND Viral Media Lab, Egyptian Organization for Biological Products & Vaccines (VACSERA), Giza, 12654, Egypt
• Khaled Ali, Department of Botany and Microbiology, Faculty of Science, Zagazig University, Zagazig, Egypt

Abstract

Antibiotic-resistant pathogenic microorganisms contaminate aquatic habitats. To prevent harmful bacteria isolated from pond and sewage water, this study assessed the green manufacturing of nanoparticles. A bioactive natural extract of Syzygium aromaticum inhibits the growth of bacteria in vitro. This study involved the isolation, purification, and identification of harmful microorganisms from aquatic sources. Traditional methods of identifying bacteria include Gram staining, bacterial cell morphology, colony shape, and biochemical assays, which are verified by 16S rRNA. The agar diffusion method is used to determine the minimum inhibitory concentration (MIC) of Syzygium aromaticum (cloves) on isolated samples. Cloves were used to biosynthesize the silver nanoparticles, and the MIC was calculated. separated from cloves for identification using gas chromatography (GC-mass) and TLC chromatography. UV, transmission electron microscopy (TEM), and the antibacterial potential against tested bacteria were used to analyze the properties of clove nanoparticles. In conclusion, 30 isolates were discovered, purified, and isolated, including Staphylococcus sciuri. By using TEM, the route of action of AgNP biosynthesis on Staphylococcus sciuri was identified. The minimum inhibitory concentration (MIC) of the antibacterial activity of the nanoparticles was statistically analyzed, and a different strategy for managing Staphylococcus sciuri was examined in comparison to the antibiotic ciprofloxacin.

Recommended Citation

Abdel-Shafi, Seham; Abdelglil, Mostafa I.; Ghaly, Mohamed F; Amer, Saida; Elsilk, Sobhy El Sawy; Mohamed, Maysa M.; and Ali, Khaled (2026) "GC-MS Analysis of Bioactive Components from Syzygium Aromaticum and its Inhibition to Pathogenic Staphylococcus Sciuri Obtained from Aquatic Sources," Al-Farahidi Biomedical Journal: Vol. 2: Iss. 1, Article 4.
Available at: https://fbmj.uoalfarahidi.edu.iq/journal/vol2/iss1/4